SMAD2 Antikörper (pSer118)

Produktnummer ABIN3032618

Der Kaninchen Polyklonal Anti-SMAD2-Antikörper wurde für ELISA, IF und DB validiert. Er ist geeignet, SMAD2 in Proben von Human zu detektieren.

Kurzübersicht für SMAD2 Antikörper (pSer118) (ABIN3032618)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Dot Blot (DB)

Produktdetails anti-SMAD2 Antikörper

Bindungsspezifität: pSer118

Kreuzreaktivität (Details): Expected species reactivity: Bovine, Chicken, Drosophila, Mouse, Pig, Rat, Zebrafish

Aufreinigung: Antigen affinity purified

Immunogen: This phospho-SMAD2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS118 of human SMAD2.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the phospho-SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:10-1:50,Dot blot: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the phospho-SMAD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Andere Bezeichnung: Smad2

Hintergrund: The protein belongs to the SMAD, a family of proteins similar to the proteins of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.

UniProt: Q15796

Pathways: Zellzyklus, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus