LC3C Antikörper (pSer12)

Produktnummer ABIN3031792

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LC3C in ELISA, WB und DB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für LC3C Antikörper (pSer12) (ABIN3031792)

Target: LC3C (MAP1LC3C) (Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LC3C Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Dot Blot (DB)

Produktdetails anti-LC3C Antikörper

Bindungsspezifität: pSer12

Kreuzreaktivität (Details): Expected species reactivity: Bovine, Mouse, Rat

Aufreinigung: Antigen affinity purified

Immunogen: This phospho-LC3C antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS12 of human LC3C.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the phospho-LC3C antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Dot blot: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the phospho-LC3C antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu LC3C

Target: LC3C (MAP1LC3C) (Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C))

Andere Bezeichnung: LC3C

Hintergrund: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

UniProt: Q9H492

Pathways: Autophagie