LC3B Antikörper (pThr12)

Produktnummer ABIN3031787

Der Kaninchen Polyklonal Anti-LC3B-Antikörper wurde für ELISA und DB validiert. Er ist geeignet, LC3B in Proben von Human zu detektieren.

Kurzübersicht für LC3B Antikörper (pThr12) (ABIN3031787)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LC3B Antikörper ist unkonjugiert

Applikation: ELISA, Dot Blot (DB)

Produktdetails anti-LC3B Antikörper

Bindungsspezifität: pThr12

Kreuzreaktivität (Details): Expected species reactivity: Bovine

Aufreinigung: Antigen affinity purified

Immunogen: This phospho-LC3B antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT12 of human LC3B.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the phospho-LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the phospho-LC3B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Andere Bezeichnung: LC3B

Hintergrund: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

UniProt: Q9GZQ8

Pathways: Autophagie