PHF1 Antikörper (N-Term)

Produktnummer ABIN3032197

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PHF1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für PHF1 Antikörper (N-Term) (ABIN3032197)

Target: PHF1 (PHD Finger Protein 1 (PHF1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PHF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-PHF1 Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Antigen affinity purified

Immunogen: This PHF1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 164-197 amino acids from the N-terminal region of human PHF1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the PHF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the PHF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu PHF1

Target: PHF1 (PHD Finger Protein 1 (PHF1))

Andere Bezeichnung: PHF1

Hintergrund: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed:18285464 and PubMed:23273982). According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed:23142980). Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.

UniProt: O43189