PGK1 Antikörper (AA 117-145)

Produktnummer ABIN3032224

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PGK1 in WB, ELISA, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für PGK1 Antikörper (AA 117-145) (ABIN3032224)

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PGK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-PGK1 Antikörper

Bindungsspezifität: AA 117-145

Kreuzreaktivität (Details): Expected species reactivity: Primate, Rat

Aufreinigung: Purified

Immunogen: A portion of amino acids 117-145 from the human protein was used as the immunogen for this PGK1 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the PGK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Andere Bezeichnung: PGK1

Hintergrund: Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process