MYLK3 Antikörper (AA 40-69)

Produktnummer ABIN3031919

Der Kaninchen Polyklonal Anti-MYLK3-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, MYLK3 in Proben von Human und Maus zu detektieren.

Kurzübersicht für MYLK3 Antikörper (AA 40-69) (ABIN3031919)

Target: MYLK3 (Myosin Light Chain Kinase 3 (MYLK3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MYLK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-MYLK3 Antikörper

Bindungsspezifität: AA 40-69

Aufreinigung: Purified

Immunogen: A portion of amino acids 40-69 from the human protein was used as the immunogen for this MYLK3 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the MYLK3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the MYLK3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu MYLK3

Target: MYLK3 (Myosin Light Chain Kinase 3 (MYLK3))

Andere Bezeichnung: MYLK3

Hintergrund: MLCK/MYLK3, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

UniProt: Q32MK0

Pathways: Regulation of Muscle Cell Differentiation