MYLK3 Antikörper (N-Term)

Produktnummer ABIN392494

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch MYLK3 in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für MYLK3 Antikörper (N-Term) (ABIN392494)

Target: MYLK3 (Myosin Light Chain Kinase 3 (MYLK3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MYLK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB3066

Produktdetails anti-MYLK3 Antikörper

Bindungsspezifität: AA 40-69, N-Term

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This MYLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human MYLK3.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-MYLK3 Antikörper (ABIN392494)

Rossi, Todd, Daniels, Bazan, Belayev: "Interferon Stimulated Gene 15 upregulation precedes the development of blood brain barrier disruption and cerebral edema after traumatic brain injury in young mice." in: Journal of neurotrauma, (2015) (PubMed).

Rossi, Ralay Ranaivo, Patel, Chrzaszcz, Venkatesan, Wainwright: "Albumin causes increased myosin light chain kinase expression in astrocytes via p38 mitogen-activated protein kinase." in: Journal of neuroscience research, Vol. 89, Issue 6, pp. 852-61, (2011) (PubMed).

Details zu MYLK3

Target: MYLK3 (Myosin Light Chain Kinase 3 (MYLK3))

Andere Bezeichnung: MYLK3

Hintergrund: MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

Molekulargewicht: 88393

Gen-ID: 91807

NCBI Accession: NP_872299

UniProt: Q32MK0

Pathways: Regulation of Muscle Cell Differentiation