MCM4 Antikörper (AA 637-665)

Produktnummer ABIN3031812

Der Kaninchen Polyklonal anti-MCM4 Antikörper (ABIN3031812) detektiert spezifisch MCM4 in WB, ELISA und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für MCM4 Antikörper (AA 637-665) (ABIN3031812)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCM4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-MCM4 Antikörper

Bindungsspezifität: AA 637-665

Kreuzreaktivität (Details): Expected species reactivity: Mouse, Xenopus

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 637-665 from the human protein was used as the immunogen for this MCM4 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the MCM4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the MCM4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Andere Bezeichnung: MCM4

Hintergrund: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

UniProt: P33991

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA