Titration of the anti-HA antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000, Immunoprecipitation: published application
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In 1X PBS pH 7.4 with 0.09 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot the anti-HA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. NSJ's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.