ALK Antikörper (AA 1517-1550)

Produktnummer ABIN3029935

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ALK in WB und ELISA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für ALK Antikörper (AA 1517-1550) (ABIN3029935)

Target: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ALK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-ALK Antikörper

Bindungsspezifität: AA 1517-1550

Aufreinigung: Antigen affinity purified

Immunogen: This Alk antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1517-1550 amino acids mouse Alk.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the Alk antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the Alk antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu ALK

Target: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Andere Bezeichnung: Alk

Hintergrund: Anaplastic lymphoma kinase is a neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. [UniProt]

UniProt: P97793

Pathways: RTK Signalweg