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Vimentin Antikörper (AA 430-457)

Name: Vimentin Antikörper (AA 430-457)
SKU: ABIN3029594
Price: 628.5 EUR
Availability: InStock

VIM Reaktivität: Human WB, IHC, ELISA, IF, FACS Wirt: Kaninchen Polyclonal unconjugated

Western blot analysis of anti-Vimentin antibody and NCI-H460 lysate (Vimentin Antikörper (AA 430-457))

Anti-Vimentin antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). (Vimentin Antikörper (AA 430-457))

FACS

Anti-Vimentin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue. (Vimentin Antikörper (AA 430-457))

IHC

Fluorescent confocal image of SY5Y cells stained with anti-Vimentin antibody at 1:100. (Vimentin Antikörper (AA 430-457))

IHC

FACS

7 Abbildungen

Produktnummer ABIN3029594

Datenblatt (PDF)

Target Alle Vimentin (VIM) Antikörper anzeigen

Vimentin (VIM)
Bindungsspezifität
32

18

16

16

16

14

11

8

8

7

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 430-457
Reaktivität
333

127

126

61

59

44

43

37

30

22

20

14

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
Human
Wirt
189

130

9

6

4

4

2
Kaninchen
Passende Sekundärantikörper
Klonalität
197

146
Polyklonal
Konjugat
200

19

13

13

11

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
Dieser Vimentin Antikörper ist unkonjugiert
Applikation
282

131

99

98

95

65

49

40

39

28

23

20

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Kreuzreaktivität (Details)

Expected species reactivity: Bovine, Chicken, Hamster, Mouse, Pig, Primate, Rat, Xenopus

Aufreinigung

Purified

Immunogen

A portion of amino acids 430-457 from the human protein was used as the immunogen for this anti-Vimentin antibody.

Isotyp

Ig Fraction

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anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reaktivität: Human, Maus, Ratte WB, IHC, ELISA, FACS Wirt: Maus Monoclonal 3A1F2 unconjugated

Applikationshinweise

The stated application concentrations are suggested starting amounts. Titration of the anti-Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:100,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot the anti-Vimentin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

Vimentin (VIM)

Andere Bezeichnung

Vimentin (VIM Produkte)

Synonyme

CTRCT30 antikoerper, cb28 antikoerper, vime antikoerper, vim antikoerper, vim1 antikoerper, vim2 antikoerper, VIM antikoerper, Vimentin antikoerper, vim4 antikoerper, vimentin antikoerper, vimentin L homeolog antikoerper, vimentin S homeolog antikoerper, VIM antikoerper, Vim antikoerper, vim antikoerper, vim.L antikoerper, vim.S antikoerper

Hintergrund

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

UniProt

P08670

Pathways

Caspase Kaskade in der Apoptose