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GAPDH Antikörper (AA 62-91)

Name: GAPDH Antikörper (AA 62-91)
SKU: ABIN3029800
Price: 628.5 EUR
Availability: InStock

GAPDH Reaktivität: Human WB, ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

Western blot analysis of lysate from A375 cell line using anti-GAPDH antibody diluted at 1:500. (GAPDH Antikörper (AA 62-91))

IHC analysis of FFPE human hepatocarcinoma tissue stained with anti-GAPDH antibody (GAPDH Antikörper (AA 62-91))

IHC

Anti-GAPDH antibody confocal immunofluorescent analysis with HeLa cell. (GAPDH Antikörper (AA 62-91))

Confocal immunofluorescent analysis of anti-GAPDH antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (GAPDH Antikörper (AA 62-91))

IHC

Western blot analysis of lysate from HeLa, HUVEC cell line (left to right) using anti-GAPDH antibody diluted at 1:1000 for each lane. (GAPDH Antikörper (AA 62-91))

Western blot analysis of anti-GAPDH antibody and 1) A2058, 2) A375, and 3) CEM lysate (GAPDH Antikörper (AA 62-91))

10 Abbildungen

Produktnummer ABIN3029800

Datenblatt (PDF)

Target Alle GAPDH Antikörper anzeigen

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Bindungsspezifität
37

21

13

9

7

6

6

5

4

4

3

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 62-91
Reaktivität
211

142

135

75

65

55

36

28

23

21

20

19

18

16

12

12

11

10

9

8

6

4

4

4

4

3

3

3

3

3

2

2

1

1

1

1

1
Human
Wirt
158

97

17

10

4
Kaninchen
Passende Sekundärantikörper
Klonalität
179

105
Polyklonal
Konjugat
155

35

22

16

5

5

5

5

4

4

4

3

3

3

3

3

2

2

2

1

1

1

1

1
Dieser GAPDH Antikörper ist unkonjugiert
Applikation
233

97

72

69

46

41

35

30

16

16

15

15

8

6

5

4

4

3

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Aufreinigung

Purified

Immunogen

A portion of amino acids 62-91 from the human protein was used as the immunogen for this anti-GAPDH antibody.

Isotyp

Ig Fraction

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GAPDH Reaktivität: Human, Maus, Ratte WB, IF, IHC (p) Wirt: Maus Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH Reaktivität: Human, Ratte WB, ELISA, IHC, IF Wirt: Ziege Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 233-259), (C-Term) antibody
GAPDH Reaktivität: Human WB, IF, IHC (p), FACS Wirt: Kaninchen Polyclonal RB16543 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF Wirt: Ziege Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH Reaktivität: Human, Maus, Ratte WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH Reaktivität: Human WB, IF, IHC (p) Wirt: Kaninchen Polyclonal RB16537 unconjugated

Applikationshinweise

The stated application concentrations are suggested starting amounts. Titration of the anti-GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:500-1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot the anti-GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung

GAPDH (GAPDH Produkte)

Synonyme

G3PD antikoerper, GAPD antikoerper, Gapd antikoerper, BEST:GH12586 antikoerper, CG12055 antikoerper, Dmel\\CG12055 antikoerper, GA3PDH antikoerper, GADPH antikoerper, GAP antikoerper, GAPDH antikoerper, GAPDH I antikoerper, GAPDH-1 antikoerper, GAPDH1 antikoerper, GAPDHI antikoerper, Gapdh antikoerper, Gapdh-1 antikoerper, Gapdh43E antikoerper, gadph antikoerper, gapdh antikoerper, gapdh-1 antikoerper, gh12586 antikoerper, cb609 antikoerper, gapd antikoerper, mg:bb02e05 antikoerper, wu:fb33a10 antikoerper, wu:ft80f05 antikoerper, KNC-NDS6 antikoerper, g3pd antikoerper, G3PDH antikoerper, glyceraldehyde-3-phosphate dehydrogenase antikoerper, Glyceraldehyde 3 phosphate dehydrogenase 1 antikoerper, glyceraldehyde-3-phosphate dehydrogenase S homeolog antikoerper, glyceraldehyde-3-phosphate dehydrogenase, type I antikoerper, olfactory receptor 8K3 antikoerper, GAPDH antikoerper, Gapdh antikoerper, Gapdh1 antikoerper, gapdh antikoerper, gapdh.S antikoerper, gapDH antikoerper, LOC100404960 antikoerper, LOC614985 antikoerper

Hintergrund

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

UniProt

P04406