ENO2/NSE Antikörper (AA 416-433)

Produktnummer ABIN4949836

Der Maus Monoklonal Anti-ENO2/NSE-Antikörper wurde für WB, IF, FACS und IHC (p) validiert. Er ist geeignet, ENO2/NSE in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für ENO2/NSE Antikörper (AA 416-433) (ABIN4949836)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ENO2/NSE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: ENO2-1375

Produktdetails anti-ENO2/NSE Antikörper

Bindungsspezifität: AA 416-433

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,IF: 1-2 μg/mL,IHC (FFPE): 0.1-0.2 μg/mL for 30 min at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Andere Bezeichnung: NSE / Neuron Specific Enolase

Hintergrund: Recognizes a protein of about 50  kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Gen-ID: 2026