CD3 epsilon Antikörper

Produktnummer ABIN4949512

Der Maus Monoklonal anti-CD3 epsilon Antikörper wird verwendet zum Nachweis von CD3 epsilon in Proben von Human und Affe. Er wurde validiert für FACS, IF und Act.

Kurzübersicht für CD3 epsilon Antikörper (ABIN4949512)

Target: CD3 epsilon (CD3E)

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Activation (Act)

Klon: CRIS-7

Produktdetails anti-CD3 epsilon Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Stimulated human leukocytes were used as the immunogen for the CD3e antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CD3e antibody should be determined by the researcher.\. Functional Activity: Induce T cell activation and proliferation (Order SAF formulation),Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3e

Hintergrund: Recognizes the epsilon-chain of CD3 (Workshop V, Code: CD03.09), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway