Rekombinanter Keratin Basic Antikörper

Produktnummer ABIN4949251

Der Kaninchen Monoklonal Anti--Antikörper wurde für WB, IF, FACS und IHC (p) validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Keratin Basic Antikörper (ABIN4949251)

Target: Keratin Basic

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: KRTH-1576R

Produktdetails

Aufreinigung: Protein A affinity chromatography

Immunogen: Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Antigendetails

Target: Keratin Basic

Andere Bezeichnung: Basic Cytokeratin

Hintergrund: This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 52  kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40  kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.