Iba1 Antikörper (C-Term)

Produktnummer ABIN492698

Der Ziege Polyklonal Anti-Iba1-Antikörper wurde für WB, IHC (p) und EIA validiert. Er ist geeignet, Iba1 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für Iba1 Antikörper (C-Term) (ABIN492698)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reaktivität: Human, Ratte, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Iba1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-Iba1 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody recognizes Allograft Inflammatory Factor-1 (AIF1).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Bovine, Goat, Hamster, Rabbit, Pig, Horse, MonkeySpecies reactivity (tested):Human and Rat

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide (C-TGPPAKKAISELP) from C-terminus of Human AIF1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/128000. Western blot: 1-3 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This antibody was validated for use in Immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by AlkalinePhosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody in aliquots at-20 °C.

Details zu Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Andere Bezeichnung: AIF1

Hintergrund: Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

Gen-ID: 199

NCBI Accession: NP_001614

UniProt: P55008

Pathways: Smooth Muscle Cell Migration