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LC3B Antikörper (N-Term)

Dieses Anti-LC3B-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von LC3B in WB und IF. Geeignet für Human, Maus, Ratte, Hamster und Affe.
Produktnummer ABIN492615

Kurzübersicht für LC3B Antikörper (N-Term) (ABIN492615)

Target

Alle LC3B (MAP1LC3B) Antikörper anzeigen
LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reaktivität

  • 112
  • 47
  • 32
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Human, Maus, Ratte, Hamster, Affe

Wirt

  • 105
  • 13
  • 7
Maus

Klonalität

  • 107
  • 19
Monoklonal

Konjugat

  • 79
  • 10
  • 8
  • 6
  • 6
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser LC3B Antikörper ist unkonjugiert

Applikation

  • 73
  • 53
  • 33
  • 32
  • 22
  • 15
  • 12
  • 10
  • 8
  • 5
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF)

Klon

2G6
  • Bindungsspezifität

    • 14
    • 13
    • 10
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Spezifität

    This antibody specifically recognizes both forms of endogenous LC3, the cytoplasmic LC3-I (18 kDa) as well as the lipidated form generated during autophagosome and autophagolysosome formation: LC3-II (16 kDa).Aff. Const.:Human, Mouse, Rat, Monkey and Hamster.

    Produktmerkmale

    Synonyms: MAP1LC3B, MAP1A/MAP1B, Map1lc3b, Map1alc3, Map1lc3, Microtubule-associated protein1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B,Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A /1B light chain 3 B

    Aufreinigung

    Subsequent Ultrafiltration and Size Exclusion Chromatography.

    Bestandteile

    incl. positive Control

    Immunogen

    Synthetic peptide hemocyanin conjugated derived from the N-terminus of LC3-B

    Isotyp

    IgG1
  • Applikationshinweise

    Immunoblotting: 0.5 μg/mL for HRPO/ECL detectionRecommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. We strongly recommend to use PVDF membranes for immunoblot analysis. Immunocytochemistry: Use at 1-10 μg/mL (Paraformaldehyd/Methanol fixation). Included Positive Control: Cell lysate from untreated Neuro 2A (See Protocols).

    Protokoll

    Positive Control: Cell lysate from untreated Neuro 2A cells, brain endothelioma (Mouse)Format: Lyophilized cell lysate from serum starved Neuro 2A. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    0.1 mg/mL

    Buffer

    PBS containing 0.09 % Sodium Azide, PEG and Sucrose/50 % Glycerol

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.

    Haltbarkeit

    12 months
  • Target

    LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

    Andere Bezeichnung

    LC3B

    Hintergrund

    Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (microtubule-associated protein1 light chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.Synonyms: Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A / 1B light chain 3 B, MAP1A/MAP1B, MAP1LC3B, Map1alc3, Map1lc3, Map1lc3b, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B

    Gen-ID

    81631

    UniProt

    Q9GZQ8

    Pathways

    Autophagie
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