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PSMA Antikörper (FITC)

FOLH1 Reaktivität: Human FACS Wirt: Maus Monoclonal 107-1A4 FITC
Produktnummer ABIN492597
  • Target Alle PSMA (FOLH1) Antikörper anzeigen
    PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))
    Reaktivität
    • 75
    • 11
    • 9
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 40
    • 36
    • 1
    Maus
    Klonalität
    • 40
    • 37
    Monoklonal
    Konjugat
    • 50
    • 5
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PSMA Antikörper ist konjugiert mit FITC
    Applikation
    • 43
    • 31
    • 30
    • 17
    • 13
    • 12
    • 12
    • 8
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Spezifität
    This antibody reacts with PSMA (100 kDa) on Flow Cytometry.
    Kreuzreaktivität (Details)
    Species reactivity (tested):Human.
    Produktmerkmale
    Synonyms: Glutamate carboxypeptidase 2, Folate hydrolase 1, Prostate-specific membrane antigen,FOLH, NAALAD1, PSM, GCP2, NAALAdase, Glutamate carboxypeptidase II, Membraneglutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I,Pteroylpoly-gamma-glutamate carboxypeptidase, Folylpoly-gamma-glutamatecarboxypeptidase
    Aufreinigung
    Protein-A Agarose Chromatography of hybridoma supernatant.
    Immunogen
    LNCaP cell homogenate.
    Klon
    107-1A4
    Isotyp
    IgG1
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  • Applikationshinweise
    Flow Cytometry: 10 μg/mL (final concentration). Positive Control: LNCaP cell. Detailed procedure is provided in Protocols.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    Flow Cytometric analysis for for floating cellsWe usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Detach the cells from culture dish by using cell dissociation buffer2) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS)and 0. 1% NaN]. 3) Resuspend the cells with washing buffer (5x10e6 cells/mL). 4) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 5) Add 20 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3or 20 µL of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 6) Add 30 µL of the primary antibody at the concentration of as suggest in theAPPLICATIONS diluted in the washing buffer. Mix well and incubate for 30 minutes at roomtemperature. 7) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 8) Add 30 µL of 1: 100 FITC conjugated anti-mouse IgG diluted with the washing buffer. Mixwell and incubate for 15 minutes at room temperature. 9) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 10) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control: LNCaP
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.5 mg/mL
    Buffer
    PBS containing 1 % BSA and 0.09 % Sodium Azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C.
    Shelf life: one year from despatch.
    Haltbarkeit
    12 months
  • Gorges, Riethdorf, von Ahsen, Nastał Y, Röck, Boede, Peine, Kuske, Schmid, Kneip, König, Rudolph, Pantel: "Heterogeneous PSMA expression on circulating tumor cells: a potential basis for stratification and monitoring of PSMA-directed therapies in prostate cancer." in: Oncotarget, Vol. 7, Issue 23, pp. 34930-41, (2018) (PubMed).

  • Target
    PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))
    Andere Bezeichnung
    PSMA / FOLH1 (FOLH1 Produkte)
    Synonyme
    FGCP antikoerper, FOLH antikoerper, GCP2 antikoerper, GCPII antikoerper, NAALAD1 antikoerper, NAALAdase antikoerper, PSM antikoerper, PSMA antikoerper, mGCP antikoerper, mopsm antikoerper, Naalad antikoerper, putative N-acetylated-alpha-linked acidic dipeptidase antikoerper, folate hydrolase 1B antikoerper, folate hydrolase 1 antikoerper, folate hydrolase (prostate-specific membrane antigen) 1 antikoerper, LOC451185 antikoerper, FOLH1B antikoerper, FOLH1 antikoerper, Folh1 antikoerper
    Hintergrund
    PSMA is a type II transmembrane protein expressed at high levels in prostate cancer and in tumor-associated neovasculature. PSMA is also known as a glutamate carboxypeptidase II (EC 3.4.17.21) that hydrolyzes the neuropeptide, N-acetyl-L-aspartyl-L-glutamate, releasing glutamate, the dominant excitatory neurotransmitter/neuromodulator of the mammalian central nervous system. The ability of PSMA to liberate g-glutamate may allow the removal of glutamate residues from dietary folates, which are unable to be transported into cells in their poly-g-glutamate form. Overexpression of PSMA in prostate cancer cells may represent an advantageous adaptation that allows the uptake of folates required for rapid division. The exopeptidase activity exhibited by PSMA and its high-level overexpression in prostate cancer cells make it a potential target for selective anticancer therapy and prodrug activation.Synonyms: FOLH, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, GCP2, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, NAALAD1, NAALAdase, PSM, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase
    Gen-ID
    2346
    UniProt
    Q04609
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