Endonuclease G Antikörper (AA 51-140)

Produktnummer ABIN492521

Der Maus Monoklonal Anti-Endonuclease G-Antikörper wurde für WB und EIA validiert. Er ist geeignet, Endonuclease G in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für Endonuclease G Antikörper (AA 51-140) (ABIN492521)

Target: Endonuclease G (ENDOG)

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Endonuclease G Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: 7G1C10

Produktdetails anti-Endonuclease G Antikörper

Bindungsspezifität: AA 51-140

Spezifität: Recognizes Endonuclease G (EndoG)

Produktmerkmale: Synonyms: Endo-G, Endonuclease G mitochondrial

Aufreinigung: Immunoaffinity Chromotography.

Immunogen: Recombinant protein corresponding to amino acids 51–140 of Human EndoG.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: EndoG antibody can be used for detection of EndoG at 2.5-5 μg/mL. Positive Controls: HepG2 cell lysate, EndoG Recombinant Protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Endonuclease G

Target: Endonuclease G (ENDOG)

Andere Bezeichnung: Endonuclease G

Hintergrund: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.Synonyms: ENDOG

Gen-ID: 2021

UniProt: Q14249

Pathways: Apoptose