CAMK2A Antikörper

Produktnummer ABIN492419

Der Maus Monoklonal anti-CAMK2A Antikörper wird verwendet zum Nachweis von CAMK2A in Proben von Human, Maus, Ratte und Rind (Kuh). Er wurde validiert für WB, IF, IP, EIA, RIA und IHC (fro).

Kurzübersicht für CAMK2A Antikörper (ABIN492419)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CAMK2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 6G9

Produktdetails anti-CAMK2A Antikörper

Kreuzreaktivität (Details): Species reactivity (tested):Mouse, Rat, Bovine, Human.

Produktmerkmale: Synonyms: CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-IIsubunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain

Aufreinigung: Protein G Chromatography

Immunogen: Partially purified Rat CaMKII

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: RIA. ELISA. Immunflourescence. Immunoprecipitation. Immunohistochemistry. Western blot: 0.1 μg/mL of this CamKIIA antibody was needed for detection of CaMKII in 20 μg of Rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-MouseIgG: AP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS pH 7.4 containing 0.09 % Sodium Azide in 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Andere Bezeichnung: CAMK2A

Hintergrund: CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca 2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968

Gen-ID: 25400

UniProt: P11275

Pathways: WNT Signalweg, Interferon-gamma Pathway, Myometrial Relaxation and Contraction