ERBB3 Antikörper (C-Term) (Biotin)

Produktnummer ABIN492350

Der Maus Monoklonal Anti-ERBB3-Antikörper wurde für WB validiert. Er ist geeignet, ERBB3 in Proben von Human zu detektieren.

Kurzübersicht für ERBB3 Antikörper (C-Term) (Biotin) (ABIN492350)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ERBB3 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB)

Klon: 11A4

Produktdetails anti-ERBB3 Antikörper (Biotin)

Bindungsspezifität: C-Term

Spezifität: This antibody specifically recognizes the C-terminus of erbB3 at 185 kDa.

Produktmerkmale: Synonyms: ERBB-3, c-erbB-3, HER-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cellsurface receptor HER3, Proto-oncogene-like protein c-ErbB-3

Aufreinigung: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Bestandteile: incl. positive Control

Immunogen: Synthetic petide conjugated to KLH. Epitope: C-terminus

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SW620 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control Provided. Cell lysate from untreated SW620Description: Cell lysate from untreated SW620 cells, colorectal adenocarcinoma (Human)Format: Lyophilized cell lysate from starved cells SW 620 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, 0.09 % Sodium Azide/PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Aliquote and freeze in liquid nitrogenAntibody can be stored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.

Haltbarkeit: 12 months

Details zu ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Andere Bezeichnung: ERBB3 / HER3

Hintergrund: ErbB3 is a member of the EGFR/erbB receptor tyrosine kinase family. ErbB3 lacks intrinsic kinase activity due to an amino acid substitution within the ATP binding site. Upon heterodimerization with the other erbB family members, erbB3 becomes phosphorylated at multiple tyrosine residues that represent docking sites for downstream signaling proteins, e.g. PI3K, grb7 and shc. The erbB3 cytoplasmic domain contains 6 docking sites for PI3K. Thus, erbB3 is a potent activator of the PI3K/PKB signaling pathway.Synonyms: ERBB-3, HER-3, Proto-oncogene-like protein c-ErbB-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cell surface receptor HER3, c-erbB-3

Gen-ID: 2065

UniProt: P21860

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung