Nucleolin Antikörper (pThr84)

Produktnummer ABIN4889383

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch Nucleolin in WB, IF und IP. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Nucleolin Antikörper (pThr84) (ABIN4889383)

Target: Nucleolin (NCL)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nucleolin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-Nucleolin Antikörper

Bindungsspezifität: pThr84

Spezifität: Reacts with Nucleolin with phoshothreonine 84 (pT84) from human and mouse

Kreuzreaktivität (Details): Cross reacts with phoshothreonine 106 (pT106). Do not cross react with phoshothreonine 76 & 92 (pT76 and pT92). The detection of P-NCL can be done during mitosis or in G2 phase, no signal in G1S phase.

Aufreinigung: Purified (protein A)

Immunogen: Synthetic peptide of human Nucleolin with a phoshothreonine at position 84

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
- WB : 1/200 to 1/2000 - IF: 1/200 to 1/1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Must be reconstituted in distilled water.

Konzentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Haltbarkeit: 6 months

Details zu Nucleolin

Target: Nucleolin (NCL)

Andere Bezeichnung: Nucleolin

Hintergrund: Ucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Gen-ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization