Nucleolin Antikörper (pThr84)

Produktnummer ABIN4889383

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Nucleolin in WB, IF und IP. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Nucleolin Antikörper (pThr84) (ABIN4889383)

Target: Nucleolin (NCL)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nucleolin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-Nucleolin Antikörper

Bindungsspezifität: pThr84

Spezifität: Reacts with Nucleolin with phoshothreonine 84 (pT84) from human and mouse

Kreuzreaktivität (Details): Cross reacts with phoshothreonine 106 (pT106). Do not cross react with phoshothreonine 76 & 92 (pT76 and pT92). The detection of P-NCL can be done during mitosis or in G2 phase, no signal in G1S phase.

Aufreinigung: Purified (protein A)

Immunogen: Synthetic peptide of human Nucleolin with a phoshothreonine at position 84

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
- WB : 1/200 to 1/2000 - IF: 1/200 to 1/1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Must be reconstituted in distilled water.

Konzentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Haltbarkeit: 6 months

Details zu Nucleolin

Target: Nucleolin (NCL)

Andere Bezeichnung: Nucleolin

Hintergrund: Ucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Gen-ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization