Phosphotyrosine Antikörper

Produktnummer ABIN488674

Dieser Maus Monoklonal Antikörper detektiert spezifisch Phosphotyrosine in WB, IP, IF und IHC. Es wurde in 1 Publikation zitiert.

Kurzübersicht für Phosphotyrosine Antikörper (ABIN488674)

Target: Phosphotyrosine

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: G104

Produktdetails anti-Phosphotyrosine Antikörper

Spezifität: Antibody reacts with phosphotyrosine and detects the presence ofphosphotyrosine in proteins of both unstimulated and stimulated cell lysated.

Kreuzreaktivität (Details): Does not cross react with phosphoserine or phosphothreonine

Homologie: antibody is not species specific

Aufreinigung: affinity purified

Immunogen: Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3-™-dimentrylaminopropyl) carbodiimide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: 1 : 1000 with standard ECL (WB)

Kommentare:

1 μg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 μg of rat tissue lysate by colorimetric immunoblot analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: protein G purified IgG1 in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of thetubes.

Lagerung: -20 °C

Informationen zur Lagerung: store at -20°C for one year, make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Haltbarkeit: 12 months

Referenzen für anti-Phosphotyrosine Antikörper (ABIN488674)

Garton, Tonks: "Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST." in: The Journal of biological chemistry, Vol. 274, Issue 6, pp. 3811-8, (1999) (PubMed).

Details zu Phosphotyrosine

Target: Phosphotyrosine

Substanzklasse: Amino Acid

Hintergrund: Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.