FGF21 Antikörper (AA 29-209)

Produktnummer ABIN4886586

Dieses Anti-FGF21-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von FGF21 in WB und ELISA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für FGF21 Antikörper (AA 29-209) (ABIN4886586)

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FGF21 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-FGF21 Antikörper

Bindungsspezifität: AA 29-209

Verwendungszweck: Anti-FGF21 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-FGF21 Antibody Picoband® (ABIN4886586). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human FGF21 recombinant protein (Position: H29-S209). Human FGF21 shares 80.7% amino acid (aa) sequence identity with mouse FGF21.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Galman, C., Lundasen, T., Kharitonenkov, A., Bina, H. A., Eriksson, M., Hafstrom, I., Dahlin, M., Amark, P., Angelin, B., Rudling, M. The circulating metabolic regulator FGF21 is induced by prolonged fasting and PPAR-alpha activation in man. Cell Metab. 8: 169-174, 2008. 2. Hondares, E., Rosell, M., Gonzalez, F. J., Giralt, M., Igelsias, R., Villarroya, F. Hepatic FGF21 expression is induced at birth via PPAR-alpha in response to milk intake and contributes to thermogenic activation of neonatal brown fat. Cell Metab. 11: 206-212, 2010. 3. Nishimura, T., Nakatake, Y., Konishi, M., Itoh, N. Identification of a novel FGF, FGF-21, preferentially expressed in the liver. Biochim. Biophys. Acta 1492: 203-206, 2000.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu FGF21

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Andere Bezeichnung: FGF21

Hintergrund:

Synonyms: Fibroblast growth factor 21,FGF-21,FGF21,UNQ3115/PRO10196,

Tissue Specificity: Expressed in fetal brain, cartilage, retina, and adult gall bladder. .

Background: FGF21 (Fibroblast growth factor 21) is a protein that in humans is encoded by the FGF21 gene, which is also a member of the fibroblast growth factor (FGF) family. The FGF21 gene is mapped on 19q13.33. Using RT-PCR, Fgf21 was expressed in several types of adipose tissue in mice, including subcutaneous and epididymal fat pads and brown adipose tissue. The level of Fgf21 expression in adipose tissue was comparable to that in liver. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Changes in Fgf21 expression due to suckling or nutritional manipulations were associated with changes in circulating free fatty acid and ketone body levels. In differentiated mouse brown adipocytes in culture, Fgf21 treatment increased the expression of thermogenic genes, caused higher total and uncoupled respiration, and enhanced glucose oxidation.

Molekulargewicht: 22 kDa

Gen-ID: 26291

Pathways: RTK Signalweg