CA3 Antikörper (AA 14-235)

Produktnummer ABIN4886494

Produktdetails anti-CA3 Antikörper

Target: CA3 (Carbonic Anhydrase III (CA3))

Bindungsspezifität: AA 14-235

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: Anti-Carbonic Anhydrase III/CA3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Carbonic Anhydrase III/CA3 Antibody Picoband® (ABIN4886494). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human CA3 recombinant protein (Position: D14-E235). Human CA3 shares 92.3% and 91.4% amino acid (aa) sequence identity with mouse and rat CA3, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Edwards, Y. H., Lloyd, J. C., Parkar, M., Povey, S.The gene for human muscle specific carbonic anhydrase (CAIII) is assigned to chromosome 8.Ann. Hum. Genet. 50: 41-47, 1986. 2. Heath, R., Carter, N. D., Jeffery, S., Edwards, R. J., Watts, D. C., Watts, R. L.Evaluation of carrier detection of Duchenne muscular dystrophy using carbonic anhydrase III and creatine kinase.Am. J. Med. Genet. 21: 291-296, 1985. 3. Wade, R., Gunning, P., Eddy, R., Shows, T., Kedes, L.Nucleotide sequence, tissue-specific expression, and chromosome location of human carbonic anhydrase III: the human CAIII gene is located on the same chromosome as the closely linked CAI and CAII genes.Proc. Nat. Acad. Sci. 83: 9571-9575, 1986.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CA3

Target: CA3 (Carbonic Anhydrase III (CA3))

Andere Bezeichnung: CA3 (CA3 Produkte)

Hintergrund:

Synonyms: Carbonic anhydrase 3,4.2.1.1,Carbonate dehydratase III,Carbonic anhydrase III,CA-III,CA3,

Tissue Specificity: Muscle specific.

Background: Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.

Molekulargewicht: 29 kDa

Gen-ID: 761

UniProt: P07451