BCL2L1 Antikörper (Middle Region)

Produktnummer ABIN4886480

Der Kaninchen Polyklonal Anti-BCL2L1-Antikörper wurde für WB, IHC, FACS und ICC validiert. Er ist geeignet, BCL2L1 in Proben von Human zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für BCL2L1 Antikörper (Middle Region) (ABIN4886480)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BCL2L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-BCL2L1 Antikörper

Bindungsspezifität: AA 75-105, Middle Region

Verwendungszweck: Anti-Bcl-X/BCL2L1 Antibody Picoband®

Sequenz: LDAREVIPMA AVKQALREAG DEFELRYRRA F

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Bcl-X/BCL2L1 Antibody Picoband® (ABIN4886480). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Bcl-X, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: BCL2L1 BCL2-like 1". 2. Boise LH, González-García M, Postema CE, Ding L, Lindsten T, Turka LA, Mao X, Nuñez G, Thompson CB (Aug 1993). "bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death". Cell 74(4): 597-608.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-BCL2L1 Antikörper (ABIN4886480)

Cui, Li, Xu, Zhang, Sun, Chen: "Emodin alleviates severe acute pancreatitis-associated acute lung injury by decreasing pre-B-cell colony-enhancing factor expression and promoting polymorphonuclear neutrophil apoptosis." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 5121-5128, (2018) (PubMed).

Zhou, Zhang, Li, Hao, Liu, Wang: "Azithromycin synergistically enhances anti-proliferative activity of vincristine in cervical and gastric cancer cells." in: Cancers, Vol. 4, Issue 4, pp. 1318-32, (2013) (PubMed).

Details zu BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Andere Bezeichnung: BCL2L1

Hintergrund:

Synonyms: Bcl-2-like protein 1,Bcl2-L-1,Apoptosis regulator Bcl-X,BCL2L1,BCL2L, BCLX,

Tissue Specificity: Bcl-X (S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X (L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Background: Bcl-2-like protein 1, also known as Bcl-X, is a protein that in humans is encoded by the BCL2L1 gene. The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.

Molekulargewicht: 29 kDa, 60 kDa

Gen-ID: 598

UniProt: Q07817

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling