AGO1 Antikörper (N-Term)

Produktnummer ABIN488536

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch AGO1 in WB, IF, ChIP und IL. Es zeigt Reaktivität gegenüber Proben von Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth) und Nicotiana benthamiana.Es wurde in 1 Publikation zitiert.

Kurzübersicht für AGO1 Antikörper (N-Term) (ABIN488536)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reaktivität: Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth), Nicotiana benthamiana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AGO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunolocalization (IL)

Produktdetails anti-AGO1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-AGO1 | Argonaute 1

Spezifität: Antibody binds microRNA and tasiRNAs, preference for 21ntmiRNAs with 5'U

Kreuzreaktivität (Details): Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana
Not reactive in: Chlamydomonas reinhardtii, Medicago sativa, Salvia sp., Spinacia oleracea, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea maysMammals

Homologie: Brassica pekinensis,Capsella rubella,Cucumis sativus,Glycine max,Malus domestica,Pisum sativum,Ricinus communis,Solanum lycopersicum,Solanum tuberosum,Vitis vinifera

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conjugated

Anwendungsinformationen

Applikationshinweise: 2 μg (ChIP), 1: 200 (IF), (IL), small-RNA-IP-Seq, 1 : 5000-1 : 10 000 (WB)

Kommentare:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-AGO1 Antikörper (ABIN488536)

Speth, Willing, Rausch, Schneeberger, Laubinger: "RACK1 scaffold proteins influence miRNA abundance in Arabidopsis." in: The Plant journal : for cell and molecular biology, Vol. 76, Issue 3, pp. 433-45, (2013) (PubMed).

Details zu AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Andere Bezeichnung: AGO1

Hintergrund:

Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Additional information: Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.To detect AGO1 in Nicotiana benthamiana, please inquire.Recommended for detection of AGO1: extreme low femtogram range chemiluminescent detection reagent

Molekulargewicht: 116.4 | 130 kDa

Gen-ID: 841262

UniProt: O04379

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization