AGO1 Antikörper (N-Term)

Produktnummer ABIN488536

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch AGO1 in WB. Er zeigt eine Reaktivität gegenüber Arabidopsis thaliana und Nicotiana benthamiana und wurde in 1 Publikation erwähnt.

Kurzübersicht für AGO1 Antikörper (N-Term) (ABIN488536)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reaktivität: Arabidopsis thaliana, Nicotiana benthamiana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AGO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-AGO1 Antikörper

Bindungsspezifität: N-Term

Spezifität: Antibody binds microRNA and tasiRNAs, preference for 21ntmiRNAs with 5'U

Kreuzreaktivität (Details): Not reactive in: Chlamydomonas reinhardtii

Homologie: Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 116.4 / 130 kDa
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.

Aufreinigung: serum

Immunogen: N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410

Anwendungsinformationen

Applikationshinweise: 1: 5000 - 1: 10 000 (WB)

Kommentare:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water.

Handhabung: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-AGO1 Antikörper (ABIN488536)

Speth, Willing, Rausch, Schneeberger, Laubinger: "RACK1 scaffold proteins influence miRNA abundance in Arabidopsis." in: The Plant journal : for cell and molecular biology, Vol. 76, Issue 3, pp. 433-45, (2013) (PubMed).

Details zu AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Andere Bezeichnung: AGO1

Hintergrund: AGI Code: At1g48410
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Molekulargewicht: expected: 116.4 kDa, apparent: 130 kDa

UniProt: O04379

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization