Rhodopsin Antikörper (N-Term)

Produktnummer ABIN487510

Dieser Maus Monoklonal Antikörper detektiert spezifisch Rhodopsin in WB, IP, IF, IHC (fro) und EIA.

Kurzübersicht für Rhodopsin Antikörper (N-Term) (ABIN487510)

Target: Rhodopsin (RHO)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Rhodopsin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 4D2

Produktdetails anti-Rhodopsin Antikörper

Bindungsspezifität: N-Term

Spezifität: N-terminus of Rhodopsin.

Kreuzreaktivität (Details): Species reactivity (tested):All Mammalian species (Human, Mouse, Rat, Bovine, Pig, etc), Most Fish, Birds, Amphibians (Frogs, Xenopus), Anchovies. Not Drosophila or other invertebrates.

Produktmerkmale: Synonyms: RHO, Opsin-2, OPN2

Aufreinigung: Protein G Chromatography.

Immunogen: Bovine Rhodopsin.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 1/1000 (1)1 μg/mL was sufficient for detection of Rhodopsin in 10 μg of Rat eye lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1-2 μg per 100-500 μg of proteinImmunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Rhodopsin

Target: Rhodopsin (RHO)

Andere Bezeichnung: Rhodopsin

Substanzklasse: Chemical

Hintergrund: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).Synonyms: OPN2, Opsin-2, RHO

Gen-ID: 6010

UniProt: P08100

Pathways: WNT Signalweg, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction