Phosphotyrosine Antikörper (phosphorylated)

Produktnummer ABIN487507

Dieser Maus Monoklonal Antikörper detektiert spezifisch Phosphotyrosine in WB, IP, IF und IHC (fro).

Kurzübersicht für Phosphotyrosine Antikörper (phosphorylated) (ABIN487507)

Target: Phosphotyrosine

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: G104

Produktdetails anti-Phosphotyrosine Antikörper

Bindungsspezifität: phosphorylated

Aufreinigung: Protein G Chromatography.

Immunogen: Phosphotyrosine, alanine and glyceine in a 1: 1: 1 ratio polymerized in the presence ofKeyhole Limpet Hemocyanin with 1-ethyl-3-(3?-dimentrylaminopropyl) carbodiimide.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000 (1,2).1 μg/mL of this antibody was sufficient for detection of phosphorylated tyrosine residues in10 μg of Rat tissue lysate by colorimetric Immunoblot analysis using Goat anti-Rat IgG-HRPas the secondary antibody. Immunoprecipitation (9). Immunofluorescence (2). Immunohistochemitry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Phosphotyrosine

Target: Phosphotyrosine

Substanzklasse: Amino Acid

Hintergrund: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).