Syndecan 1 Antikörper (FITC)

Produktnummer ABIN487502

Produktdetails anti-Syndecan 1 Antikörper (FITC)

Target: Syndecan 1 (SDC1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Syndecan 1 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Spezifität: Recognizes CD138/Syndecan-1.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Produktmerkmale: Synonyms: SYND1, SDC1, SDC

Aufreinigung: Protein-A Sepharose Chromatography of hybridoma supernatant.

Immunogen: XG1 and U266 myeloma cell lines. Remarks: Hybridoma was established by fusion of mouse myeloma cell Sp2/0 with Balb/cmouse splenocyte.

Klon: 15-11-14

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 10-20 μg/mL (final concentration). Positive Control: KM3, RPMI8226, U226. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Flow cytometric analysis for floating cellsProtocol 1. 1) Wash the cells 3 times with washing buffer [PBScontaining 2% fetal calf serum (FCS) and 0. 1% NaN3]. 2) Add 10 µL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 3) Add 30 µL of the FITC labeled anti Syndecan-1/CD138 monoclonal antibody (10-20µg/mL) diluted with the washing buffer. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 4) Add 1 mL of the washing buffer followed bycentrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Protocol 2. We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum and 0. 1% NaN3 (5x106cells/mL). 3) Add 20 µL of the non diluted FITC labeled anti-Syndecan-1/CD138 monoclonal antibodyinto each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all steps described below. 1) Add 20 µL of the non diluted FITC labeled anti-syndecan-1/CD138 monoclonal antibodyinto each tube. 2) Add 50 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 3) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 4) Resuspend the cells with 500 ìL of the washing buffer [PBS containing 2% fetal calfserum (FCS) and 0. 1% NaN3] and analyze by a flow cytometer. Positive controls for Flow cytometry: KM3, RPMI8226, U226

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.05 mg/mL

Buffer: PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Syndecan 1

Target: Syndecan 1 (SDC1)

Andere Bezeichnung: CD138 / Syndecan-1 (SDC1 Produkte)

Hintergrund: Syndecan-1 (CD138) is a heparan sulfate proteoglycan expressed on the surface of plasma cel1s and some epithelial cells where it mediates cell-cell and cell-extracellular matrix adhesion, and may facilitate the binding of growth factors to their high-affinity receptors as shown for basic fibroblast growth factor (b-FGF). It has been reported that altered expression of syndecan-1 in several tumors such as colorectal cancer, esophageal carcinoma, lung carcinoma, gastric cancer and multiple myeloma, and alteration of syndecan-1 expression in the tumors correlated with patients survival rate, stage, or metastasis.Synonyms: SDC, SDC1, SYND1

Gen-ID: 6382

UniProt: P18827

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism