A2B5 Antikörper

Produktnummer ABIN479509

Produktdetails

Target: A2B5

Reaktivität: Huhn, Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Recognizes chicken Neuron Cell Surface Antigen A2B5. Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Species cross-reactivity: human.

Aufreinigung: Purified

Immunogen: Chick retina cells.

Type of Immunogen: Cells

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Approved: Flo, ICC (1:100 - 1:500), IF (1:100 - 1:500), IHC, IHC-Fr

Usage: Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Indirect Immunofluorescence: 1:100-1:500. For the detection of neurons in tissue culture. Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments. Immunohistochemistry: Frozen, fixed tissues. Immunocytochemistry: Confocal fluorescent analysis of PC12 cells. Positive control: Type II astrocytes, human neural progenitors, PC12 cells.

Kommentare:

Target Species of Antibody: Chicken

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Antigendetails

Target: A2B5

Andere Bezeichnung: Neuron Cell Surface Antigen A2b5