A2B5 Antikörper

Produktnummer ABIN2688840

Produktdetails

Target: A2B5

Reaktivität: Huhn, Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), BioImaging (BI), Cytotoxicity Test (CyTox)

Marke: BD Pharmingen™

Produktmerkmale: The 105/A2B5 monoclonal antibody binds to gangliosides in neural tissue in the retina, brain, spinal cord, and dorsal root ganglia. The antigenic gangliosides are found on the plasma membrane of neural cell bodies, but not axons or dendrites. Expression levels of the gangliosides that mAb 105/A2B5 binds to, including GT3 and its O-acetylated derivative, decrease during embryonic development of the brain. Thus, mAb 105/A2B5 may be used to monitor the maturation of brain tissue. A2B5 antigens have also been associated with numerous tumors, including glioblastoma multiforme tumor cells, pancreatic islet tumor cells, and rat insulinoma cells, and are thought to contribute to chemoresistance and increased tumor proliferation and recurrence. Flow cytometric analysis of A2B5 expression on mouse pancreatic tumor (insulinoma) cells and Human embryonic stem cell (hESC)-derived neurons. Mouse Beta-TC-6 cells (ATCC, CRL-11506, left panel) or human H9 (WiCell, Madison, WI) hESC-derived neurons (right panel) were harvested with Accutase™ Cell Detachment Solution (Cat. No. 561527). The cells were stained with either Purified Mouse IgM, κ Isotype Control (Cat. No. 555581, dashed line histograms) or Purified Mouse Anti- A2B5 monoclonal antibody (solid line histograms) at matched concentrations. Dot plots were derived from gated events based on light scattering characteristics of viable cells. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-A2B5 - Purified - Clone 105/A2B5 (also known as antibody A2B5, F12A2B5, 105) - Isotype Mouse IgM - Reactivity Ms, Hu, Chick, Rat - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Chicken embryo retina Cells

Klon: 105-A2B5

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen

Balik, Mirossay, Bohus, Sulla, Mirossay, Sarissky: "Flow cytometry analysis of neural differentiation markers expression in human glioblastomas may predict their response to chemotherapy." in: Cellular and molecular neurobiology, Vol. 29, Issue 6-7, pp. 845-58, (2009) (PubMed).

Eisenbarth, Walsh, Nirenberg: "Monoclonal antibody to a plasma membrane antigen of neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, Issue 10, pp. 4913-7, (1980) (PubMed).

Antigendetails

Target: A2B5