DYNC1I1 Antikörper (N-Term)

Produktnummer ABIN472619

Der Maus Monoklonal Anti-DYNC1I1-Antikörper wurde für WB, ICC, IF und IP validiert. Er ist geeignet, DYNC1I1 in Proben von Ratte, Maus, Rind (Kuh) und Schaf zu detektieren.

Kurzübersicht für DYNC1I1 Antikörper (N-Term) (ABIN472619)

Target: DYNC1I1 (Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1))

Reaktivität: Ratte, Maus, Rind (Kuh), Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DYNC1I1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-DYNC1I1 Antikörper

Bindungsspezifität: N-Term

Spezifität: Recognizes all known forms of the Mr 74kD intermediate chain subunit (IC74) of mammalian cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes). Species cross-reactivity: Rat, mouse, bovine, sheep, fish, Drosophila and Xenopus. Does not react with squid.

Aufreinigung: Purified

Immunogen: Bovine brain cytoplasmic dynein.

Type of Immunogen: Purified protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ICC (1:50 - 1:100), IF, IP, WB (1:1000 - 1:5000)

Usage: Suitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Immunocytochemistry: 1:50-1:100. Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells. Western Blot: 1:1000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells. Immunoprecipitation: 10 μL/0.5g tissue. The monoclonal clearly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.6, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu DYNC1I1

Target: DYNC1I1 (Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1))