FZD3 Antikörper (Cysteine-Rich Domain, Extracellular) (Biotin)

Produktnummer ABIN470848

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch FZD3 in WB, IHC, FACS, ICC und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für FZD3 Antikörper (Cysteine-Rich Domain, Extracellular) (Biotin) (ABIN470848)

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser FZD3 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-FZD3 Antikörper (Biotin)

Bindungsspezifität: Cysteine-Rich Domain, Extracellular

Spezifität: Recognizes mouse Frizzled-3. Shows no cross-reactivity with rmFrizzled-1, -4, -7 and -8. Species cross-reactivity: Human.

Aufreinigung: Protein G purified

Immunogen: NS0-derived rmFrizzled-3 extracellular CRD.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo, ICC, IHC, IHC-Fr, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Detected Frizzled-3 by flow cytometry and immunohistochemistry on the differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Immunocytochemistry and flow cytometry experiments on human HEK 293 cells demonstrate that this antibody cross-reacts with human Frizzled-3. Western Blot: Use at 1-2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-3. The detection limit for rmFrizzled-3 is ~0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute this antibody to 0.2 mg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of the biotinylated monoclonal antibody may be visualized by adding streptavidin conjugated to a fluorochrome. Immunocytochemistry: Use with the appropriate secondary reagents at 8 μg/mL to detect Frizzled-3 in cells or frozen mouse embryos. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 1 mL sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS, BSA

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu FZD3

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Andere Bezeichnung: FZD3 / Frizzled 3

Hintergrund: Name/Gene ID: FZD3
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3

Gen-ID: 7976

UniProt: Q9NPG1

Pathways: WNT Signalweg, Tube Formation