M-CSF/CSF1 Antikörper (Biotin)

Produktnummer ABIN465826

Dieses Anti-M-CSF/CSF1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von M-CSF/CSF1 in WB, ELISA und Neut. Geeignet für Maus.

Kurzübersicht für M-CSF/CSF1 Antikörper (Biotin) (ABIN465826)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser M-CSF/CSF1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-M-CSF/CSF1 Antikörper (Biotin)

Spezifität: Mouse M-CSF

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine M-CSF.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mM-CSF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mM-CSF. To detect mM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine M-CSF (LS-C104811) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mM-CSF. Western Blot: To detect mM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu M-CSF/CSF1

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Andere Bezeichnung: CSF1 / CSF-1 / MCSF

Hintergrund: Name/Gene ID: CSF1
Family: Hormone

Synonyms: CSF1, CSF-1, MCSF, M-CSF, Lanimostim

Gen-ID: 1435

UniProt: P09603

Pathways: RTK Signalweg