IL-2 Antikörper (Biotin)

Produktnummer ABIN465712

Dieses Anti-IL-2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von IL-2 in WB, ELISA und Neut. Geeignet für Maus.

Kurzübersicht für IL-2 Antikörper (Biotin) (ABIN465712)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-2 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-IL-2 Antikörper (Biotin)

Spezifität: Mouse IL-2

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine IL-2.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mIL-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-2. To detect mIL-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-2 (LS-C104677) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-2. Western Blot: To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Andere Bezeichnung: IL2

Hintergrund: Name/Gene ID: IL2
Family: Interleukin

Synonyms: IL2, Aldesleukin, Interleukin 2, Lymphokine, IL-2, Interleukin-2, TCGF, T cell growth factor, T-cell growth factor

Gen-ID: 3558

UniProt: P60568

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation