GM-CSF Antikörper (Biotin)

Produktnummer ABIN465667

Dieses Anti-GM-CSF-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GM-CSF in WB, ELISA und Neut. Geeignet für Human.

Kurzübersicht für GM-CSF Antikörper (Biotin) (ABIN465667)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GM-CSF Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-GM-CSF Antikörper (Biotin)

Spezifität: Human GM-CSF

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human GM-CSF.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hGM-CSF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hGM-CSF. To detect hGM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human GM-CSF (LS-C104671) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGM-CSF. Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Andere Bezeichnung: CSF2 / GMCSF / GM-CSF

Hintergrund: Name/Gene ID: CSF2

Synonyms: CSF2, Colony-stimulating factor, CSF, GM-CSF, Sargramostim, Molgramostin, GMCSF

Gen-ID: 1437

UniProt: P04141

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin