FGF7 Antikörper

Produktnummer ABIN465624

Dieses Anti-FGF7-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von FGF7 in WB, ELISA und Neut. Geeignet für Human.

Kurzübersicht für FGF7 Antikörper (ABIN465624)

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FGF7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-FGF7 Antikörper

Spezifität: Human KGF

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human KGF.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.56 - 0.85 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 0.5 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND]of the biological activity of hKGF (25 ng/mL), a concentration of 0.56-0.85 μg/mL of this antibody is required. ELISA: To detect hKGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human KGF (LS-C104287) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hKGF. Western Blot: To detect hKGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hKGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu FGF7

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Andere Bezeichnung: FGF7 / KGF

Hintergrund: Name/Gene ID: FGF7
Family: HBGF

Synonyms: FGF7, FGF-7, Fibroblast growth factor- 7, Keratinocyte growth factor, Fibroblast growth factor 7, HBGF-7, KGF

Gen-ID: 2252

UniProt: P21781

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung