Thymic Stromal Lymphopoietin Antikörper

Produktnummer ABIN465620

Der Kaninchen Polyklonal anti-Thymic Stromal Lymphopoietin Antikörper wird verwendet zum Nachweis von Thymic Stromal Lymphopoietin in Proben von Human. Er wurde validiert für WB und ELISA.

Kurzübersicht für Thymic Stromal Lymphopoietin Antikörper (ABIN465620)

Target: Thymic Stromal Lymphopoietin (TSLP)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Thymic Stromal Lymphopoietin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Thymic Stromal Lymphopoietin Antikörper

Spezifität: Human TSLP

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TSLP.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human prostate. The recommended concentration is 0.125 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hTSLP by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TSLP (LS-C104690) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTSLP. Western Blot: To detect hTSLP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTSLP is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Thymic Stromal Lymphopoietin

Target: Thymic Stromal Lymphopoietin (TSLP)

Andere Bezeichnung: TSLP

Hintergrund: Name/Gene ID: TSLP
Family: Cytokine

Synonyms: TSLP, Thymic stromal lymphopoietin

Gen-ID: 85480

UniProt: Q969D9