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G-CSF Antikörper

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch G-CSF in WB, ELISA und Neut. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN465577

Kurzübersicht für G-CSF Antikörper (ABIN465577)

Target

Alle G-CSF (CSF3) Antikörper anzeigen
G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reaktivität

  • 125
  • 33
  • 31
  • 20
  • 3
  • 2
Human

Wirt

  • 85
  • 45
  • 15
  • 2
  • 1
Kaninchen

Klonalität

  • 75
  • 73
Polyklonal

Konjugat

  • 84
  • 13
  • 6
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser G-CSF Antikörper ist unkonjugiert

Applikation

  • 85
  • 53
  • 49
  • 22
  • 21
  • 21
  • 18
  • 14
  • 13
  • 13
  • 10
  • 8
  • 7
  • 7
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Neutralization (Neut)
  • Spezifität

    Human G-CSF

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    E.coli derived recombinant Human G-CSF.

    Type of Immunogen: Cell extract
  • Applikationshinweise

    Approved: ELISA (0.5 - 2 μg/mL), Neut (4 - 6 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hG-CSF (0.5 ng/mL), a concentration of 0.04-0.06 μg/mL of this antibody is required. ELISA: To detect hG-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF (LS-C104674) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hG-CSF. Western Blot: To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    Kommentare

    Target Species of Antibody: Human

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitute with sterile water

    Konzentration

    Lot specific

    Buffer

    Lyophilized from PBS,  pH 7.2

    Handhabung

    Avoid freeze/thaw cycles.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • Target

    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

    Andere Bezeichnung

    CSF3 / G-CSF / GCSF

    Hintergrund

    Name/Gene ID: CSF3

    Synonyms: CSF3, C17orf33, Filgrastim, G-CSF, GCSF, Pluripoietin, CSF3OS, Lenograstim

    Gen-ID

    1440

    UniProt

    P09919

    Pathways

    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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