G-CSF Antikörper
Kurzübersicht für G-CSF Antikörper (ABIN465577)
Target
Alle G-CSF (CSF3) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Spezifität
- Human G-CSF
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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E.coli derived recombinant Human G-CSF.
Type of Immunogen: Cell extract
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Applikationshinweise
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Approved: ELISA (0.5 - 2 μg/mL), Neut (4 - 6 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hG-CSF (0.5 ng/mL), a concentration of 0.04-0.06 μg/mL of this antibody is required. ELISA: To detect hG-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF (LS-C104674) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hG-CSF. Western Blot: To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Reconstitute with sterile water
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Konzentration
- Lot specific
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Buffer
- Lyophilized from PBS, pH 7.2
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Handhabung
- Avoid freeze/thaw cycles.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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Andere Bezeichnung
- CSF3 / G-CSF / GCSF
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Hintergrund
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Name/Gene ID: CSF3
Synonyms: CSF3, C17orf33, Filgrastim, G-CSF, GCSF, Pluripoietin, CSF3OS, Lenograstim -
Gen-ID
- 1440
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UniProt
- P09919
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
Target
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