Thrombopoietin Antikörper (Biotin)

Produktnummer ABIN465560

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Thrombopoietin in WB, ELISA und Neut. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Thrombopoietin Antikörper (Biotin) (ABIN465560)

Target: Thrombopoietin (THPO)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Thrombopoietin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-Thrombopoietin Antikörper (Biotin)

Spezifität: Human TPO

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TPO.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hTPO by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TPO (LS-C104802) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Thrombopoietin

Target: Thrombopoietin (THPO)

Andere Bezeichnung: THPO / TPO / Thrombopoietin

Hintergrund: Name/Gene ID: THPO
Family: Hormone

Synonyms: THPO, C-mpl ligand, MGDF, MPL ligand, THCYT1, Thrombopoietin nirs variant 1, MKCSF, MPLLG, Thrombopoietin

Gen-ID: 7066

UniProt: P40225

Pathways: JAK-STAT Signalweg, Hormone Activity