WISP2 Antikörper (Biotin)

Produktnummer ABIN465432

Der Kaninchen Polyklonal anti-WISP2 Antikörper wird verwendet zum Nachweis von WISP2 in Proben von Human. Er wurde validiert für WB und ELISA.

Kurzübersicht für WISP2 Antikörper (Biotin) (ABIN465432)

Target: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WISP2 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-WISP2 Antikörper (Biotin)

Spezifität: Human CTGFL/WISP-2

Aufreinigung: Affinity purified

Immunogen: E.coli derived recombinant Human CTGFL/WISP-2.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hCTGFL/WISP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGFL/WISP-2. To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human CTGFL/WISP-2 (LS-C104572) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL sterile PBS containing 0.1 % BSA.

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu WISP2

Target: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Andere Bezeichnung: WISP2

Hintergrund: Name/Gene ID: WISP2

Synonyms: WISP2, CCN5, CCN family member 5, CTGFL, CT58, CTGF-L, WISP-2

Gen-ID: 8839

UniProt: O76076

Pathways: WNT Signalweg, Growth Factor Binding