IL-7 Antikörper (Biotin)

Produktnummer ABIN465360

Der Kaninchen Polyklonal Anti-IL-7-Antikörper wurde für WB, ELISA und Neut validiert. Er ist geeignet, IL-7 in Proben von Human zu detektieren.

Kurzübersicht für IL-7 Antikörper (Biotin) (ABIN465360)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-7 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-IL-7 Antikörper (Biotin)

Spezifität: Human IL-7

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-7.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human IL-7 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-7. To detect Human IL-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-7 (LS-C104554) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-7. Western Blot: To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant hIL-7 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Andere Bezeichnung: IL7

Hintergrund: Name/Gene ID: IL7
Family: IL-7/ IL-9

Synonyms: IL7, IL-7, Interleukin-7, Interleukin 7

Gen-ID: 3574

UniProt: P13232

Pathways: JAK-STAT Signalweg