CD22 Antikörper (Biotin)

Produktnummer ABIN465292

Der Biotin-konjugierte Kaninchen Polyklonal anti-CD22 Antikörper (ABIN465292) detektiert spezifisch CD22 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CD22 Antikörper (Biotin) (ABIN465292)

Target: CD22 (CD22 Molecule (CD22))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD22 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CD22 Antikörper (Biotin)

Spezifität: Human sCD22

Aufreinigung: Immunoaffinity purified

Immunogen: CHO cell derived recombinant Human sCD22.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hCD22 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hCD22. To detect hCD22 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human CD22 (LS-C104507) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCD22. Western Blot: To detect hCD22 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCD22 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu CD22

Target: CD22 (CD22 Molecule (CD22))

Andere Bezeichnung: CD22

Hintergrund: Name/Gene ID: CD22
Family: Immunoglobulin

Synonyms: CD22, CD22 Molecule, CD22 antigen, SIGLEC2, SIGLEC-2, T-cell surface antigen Leu-14, B-cell receptor CD22, BL-CAM

Gen-ID: 933

UniProt: P20273