Galectin 3 Antikörper

Produktnummer ABIN464873

Der Kaninchen Polyklonal anti-Galectin 3 Antikörper wird verwendet zum Nachweis von Galectin 3 in Proben von Human. Er wurde validiert für WB und ELISA.

Kurzübersicht für Galectin 3 Antikörper (ABIN464873)

Target: Galectin 3 (LGALS3)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Galectin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Galectin 3 Antikörper

Spezifität: Human Galectin-3

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Galectin-3.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a  pH 6 sodium citrate buffer. Optimal concentrations and conditions may vary. ELISA: To detect hGalectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Galectin-3 (LS-C104371) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGalectin-3. Western Blot: To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL.

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Galectin 3

Target: Galectin 3 (LGALS3)

Andere Bezeichnung: MAC-2 / Galectin 3

Substanzklasse: Chemical

Hintergrund: Name/Gene ID: LGALS3

Synonyms: LGALS3, 35 kd lectin, 35 kDa lectin, CBP35, GAL3, Galactoside-binding protein, GALIG, IgE-binding protein, Laminin-binding protein, Gal-3, GALBP, MAC-2, Mac-2 antigen, L31, MAC2, CBP 35, Galactose-specific lectin 3, Galectin-3, L-31, Lectin L-29

Gen-ID: 3958

UniProt: P17931

Pathways: RTK Signalweg