BDNF Antikörper (AA 183-194)

Produktnummer ABIN462499

Dieser Ziege Polyklonal Antikörper detektiert spezifisch BDNF in IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus, Schwein, Pferd, Hund, Meerschweinchen, Rind (Kuh), Hamster und Affe.

Kurzübersicht für BDNF Antikörper (AA 183-194) (ABIN462499)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reaktivität: Human, Ratte, Maus, Schwein, Pferd, Hund, Meerschweinchen, Rind (Kuh), Hamster, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser BDNF Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-BDNF Antikörper

Bindungsspezifität: AA 183-194

Spezifität: Human BDNF. This antibody is expected to recognise all reported isoforms (NP_001700.2, NP_001700.2, NP_733928.1, NP_733929.1, NP_733930.1, NP_733931.1)

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Mouse, Rat, Shrew, Dolphin, Hamster, Elephant, Panda, Bovine, Dog, Cat, Horse, Pig, Guinea pig, Trout, Seabass, Stickleback (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide ETKCNPMGYTKE from an internal region of human BDNF (NP_001700.2, NP_001700.2, NP_733928.1, NP_733929.1, NP_733930.1, NP_733931.1). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Mouse, Rat, Shrew, Dolphin, Hamster, Elephant, Panda, Bovine, Dog, Cat, Horse, Pig, Guinea pig, Trout, Seabass, Stickleback (100%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Andere Bezeichnung: BDNF

Hintergrund: Name/Gene ID: BDNF
Family: NGF Beta

Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin

Gen-ID: 627

NCBI Accession: NP_001700, NP_733928, NP_733929, NP_733930, NP_733931

UniProt: P23560

Pathways: RTK Signalweg, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity