CHRNA7 Antikörper

Produktnummer ABIN462402

Der Ziege Polyklonal anti-CHRNA7 Antikörper (ABIN462402) detektiert spezifisch CHRNA7 in IHC, ELISA und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Rind (Kuh), Huhn, Affe, Hund, Schwein und Pferd.

Kurzübersicht für CHRNA7 Antikörper (ABIN462402)

Target: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Reaktivität: Human, Rind (Kuh), Huhn, Affe, Hund, Schwein, Pferd

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CHRNA7 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CHRNA7 Antikörper

Spezifität: Human CHRNA7. This antibody also reacts with CHRNA7-FAM7A fusion isoform 1 (NP_647536.1) and isoform 2 (NP_683709.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bovine, Horse, Pig, Opossum, Chicken (100%) Mouse, Rat, Hamster, Rabbit (94%) Marmoset, Xenopus, Zebrafish (88%) Bat, Platypus (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide KRPGEDKVRPACQHKQ from an internal region of human CHRNA7 (NP_000737.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bovine, Horse, Pig, Opossum, Chicken (100%), Mouse, Rat, Hamster, Rabbit (94%), Marmoset, Xenopus, Zebrafish (88%), Bat, Platypus (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3 - 6 μg/mL), Peptide-ELISA

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CHRNA7

Target: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Andere Bezeichnung: CHRNA7

Hintergrund: Name/Gene ID: CHRNA7
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel

Synonyms: CHRNA7, AChR-alpha7, Acra 7, CHRNA7-2, NAChR alpha 7, NACHRA7

Gen-ID: 1139

NCBI Accession: NP_000737

UniProt: P36544

Pathways: Synaptic Membrane