Collagen Type I Antikörper

Produktnummer ABIN462339

Produktdetails anti-Collagen Type I Antikörper

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen Type I Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Marke: IHC-plus™

Spezifität: Human collagen type. Cross-reactivity: Human collagen type I: 100 % , Human collagen type II: <0.5 %, Human collagen type III: < 25 %, Human collagen type IV: <0.5 %, Human collagen type V: <0.5 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin: <0.5 %.

Aufreinigung: Ammonium sulfate precipitation

Immunogen: Human placental collagen Type I.

Type of Immunogen: Purified protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IF, IHC, IHC-P (1:100)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3653 was determined to be 1:100. ELISA on human collagen type I: 1:4000. Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue: 1:10-1:40.

Not recommended for: WB

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.5, 0.1 % Mannitol, 0.1 % Dextran.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Andere Bezeichnung: Collagen I (COL1 Produkte)

Synonyme: collagen type III alpha 1 chain antikoerper, COL3A1 antikoerper