TDP1 Antikörper (AA 1-298)

Produktnummer ABIN462272

Der Maus Monoklonal Anti-TDP1 Antikörper wurde für WB, ELISA, IHC und IHC (p) validiert. Er ist geeignet, TDP1 in Proben von Human zu detektieren.

Kurzübersicht für TDP1 Antikörper (AA 1-298) (ABIN462272)

Target: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TDP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: AT1F2

Produktdetails anti-TDP1 Antikörper

Bindungsspezifität: AA 1-298

Spezifität: Human TDP1 (1-298aa)

Aufreinigung: Protein G purified

Immunogen: Recombinant human TDP1 (1-298aa) purified from E. coli

Type of Immunogen: Recombinant protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of MCF7 (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TDP1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Details zu TDP1

Target: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Andere Bezeichnung: TDP1

Hintergrund: Name/Gene ID: TDP1

Synonyms: TDP1, Tyr-DNA phosphodiesterase 1, SCAN1

Gen-ID: 55775

UniProt: Q9NUW8