FOXO3 Antikörper (pThr24, Thr32)

Produktnummer ABIN461993

Der Kaninchen Polyklonal Anti-FOXO3-Antikörper wurde für WB, IHC, ELISA, IHC (p) und IP validiert. Er ist geeignet, FOXO3 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für FOXO3 Antikörper (pThr24, Thr32) (ABIN461993)

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FOXO3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-FOXO3 Antikörper

Bindungsspezifität: pThr24, Thr32

Marke: IHC-plus™

Spezifität: Detects endogenous levels of FKHR (FKHRL1) only when activated by phosphorylation at Thr24 of FKHR or Thr32 of FKHRL1. Also reacts with phosphorylated AFX at Thr28. Does not react with FKHR family members phosphorylated at other sites. Species cross-reactivity: Human, mouse, rat.

Aufreinigung: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr24 of human FKHR.

Type of Immunogen: Synthetic phospho-peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (1:100), IP (1:50), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Andere Bezeichnung: FOXO3 / FOXO3A

Hintergrund: Name/Gene ID: FOXO3
Family: Transcription factor

Synonyms: FOXO3, AF6q21, AF6q21 protein, Forkhead box O3, Forkhead box O3A, FOXO3A, FKHRL1P2, Forkhead box protein O3, FOXO2, FKHRL1

Gen-ID: 2309

UniProt: O43524

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Carbohydrate Homeostasis